Smittstop is tested against the H1N1 swine influenza virus and other viruses

The tests include trade secrets, therefore some parts are not shown.
In the event of a business opportunity of a certain size, and the signing of confidentiality agreements more information can be displayed.

Test for swine flu H1N1 and herpes virus

Summary of test results for herpes virus and swine influenza H1N1-cationic polymer applied to nonwoven cellulose.

Virus: (The effect against Herpes simplex virus -1 was tested at the Virology Dept.. at (removed – trade secret) and the efficacy against H1N1, a laboratory influenza virus similar to the Avian influenza virus, was assayed at (removed – trade secret).

Antiviral effects were samples in virus-adapted ASTM E 2149-01 tests on both the herpes simplex virus (> 99% red.) and the H1N1 influenza virus (> 97% red.) In cellulose nonwoven.

Here is a test of “ordinary” influenza:

Influenza H3 – Cationic polymer

Virus: Influenza H3

HA titer: 1:32

Medium: M-199 + PEST, 1% + trypsin, 0.1% (was set up after incubaktion with the fabric)

Virus was diluted: 1:8, 1:16, 1:32 and 1:64.

From each dilution was 6 ml withrdawn to three falcontubes.
Fabric A and fabric C were in separate tubes with half cloth (0.125 g) and one tube was without fabric.
The tubes were incubated at a vibrating table for one hour.

After one hour of incubation 6 ul trypsin was set to each tube (0.1%).

All twelve tubes was diluted two-fold out to 1:64 and 1 ml of each dilution was put on MDCK cells in duplicate (24-hole plates were used).

The cells were incubated 24 hours at 37 ° C and then infection rate was read by observation of the cells in light microscope.

Orig-dilution End-

dilution

Cloth Read Cloth Read Cloth Read
1:8 1:8 A neg C 3 3
1:8 1:16 A neg C 3 3
1:8 1:32 A neg C 3 3
1:8 1:64 A neg C 2 3
1:16 1:16 A neg C 4 3
1:16 1:32 A neg C 4 3
1:16 1:64 A neg C 3 3
1:32 1:32 A neg C 4 3
1:32 1:64 A neg C 3 2
1:64 1:64 A neg C 3 2

No virus dilution that had been incubated with cloth A, gave no cell infection ie the virus seems to have lost its ability to infect cells.
However, it should be added that these tubes was a difficult read because the cells sometimes had detached from the bottom and was slightly overgrown. They, however, was interpreted as negative infection as no clear picture of the infection was found.

The virus dilutions which had contact with the fabric C or no cloth at all was sometimes difficult to read due to detached cells, but in all these tubes were in all cases, clear signs of infection in different grades (1-5 where 5 is the highest infection rate).

Information for readers on tests

Fabric (A) were treated with cationic polymer. This test will eventually (as soon as we can) become a product for the treatment of fabric / clothing.

Influenza viruses are very similar and in the same group.
The effect is is identical between all influnza viruses.
H1N1 swine influenza virus has no chance against the cationic polymer in Infectious Top.

Aerosolized bacteria, test on hard surfaces:

For this type of realistic test is there no standard, but common and well-known methods have been used.
For depositing bacteria and for quantification of viability.

Bacterial suspension is sprayed on a hard surface upon which the sample is laid to dry in room temperature (15 min).
Thereafter the viability is assayed either by an agar pressure stick that is pressed against the surface for a few seconds and then sealed or by agar overlay.

Colonies are counted after overnight incubation.

In this test, the reduction is generally over 99%, both when assayed with pressure stick and with agar overlay.

Test bacteria: S. aureus, MRSA (sjukhussjukan), P. aeruginosa, E. coli, Candida albicans, E. hirae

The above test is made by bacteria and not viruses, but the effect is the same, the protection of hard surfaces – which was what was tested. Many tests are made which are not mentioned here.

Pressure Plate Test is very easy to perform even in the real environment and it is cheap – treat a surface with Smittstop, let it be affected for a certain time and then test and compare with the corresponding untreated.

The above bacterial test is done in institutions in a laboratory but it is easy to make simple practical tests yourself.

Test plate / pressure plate can be purchased from any company that gives this type of service to the food industry – at good prices.
They can often do the reading after cultivation in an oven, or you can do it yourself, instructions are included with the plates.

The food industry use these plates to check the food hygiene and cleaning hygien/quality within their self control.